Efficient replication bypass of size-expanded DNA base pairs in bacterial cells
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Essigmann_Efficient replication.pdf
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Author(s)
Delaney, James C.
Gao, Jianmin
Liu, Haibo
Shrivastav, Nidhi
Essigmann, John M.
Kool, Eric T.
Date Issued
May 2009
Journal
Angewandte Chemie
Publisher
Wiley Blackwell
Citation
Delaney, James C. et al. “Efficient Replication Bypass of Size-Expanded DNA Base Pairs in Bacterial Cells.” Angewandte Chemie International Edition 48.25 (2009): 4524–4527.
Version
Author's final manuscript
Abstract
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
Description
Author Manuscript 2012 September 06
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/anie.200805683
