BBF RFC 47: BioBytes Assembly Standard
Name
BBF RFC 47.pdf
Description
BBF_RFC_47
Size
440.36 KB
Format
Adobe PDF
Checksum (MD5)
6288e77495a49f980ffda6321844faf9
Author(s)
Ellison, Michael
Ridgway, Doug
Fedor, Justin
Garside, Erin
Robinson, Kelly
Lloyd, David
Date Issued
October 29, 2009
Series/Report no.
BBF RFC;47
Abstract
The BioBytes assembly method provides a new high-throughput way of assembling
plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic
parts with long (12 bp) standardized sticky ends, it allows for greater speed and
efficiency in assembling these components. This document defines a standard for genetic
parts compatible with the assembly system and describes detailed protocols for the
construction of such parts and their rapid assembly. Although the original motivation for
the method was to produce whole synthetic genomes, the design allows for any type of
DNA construction.
Subjects
synthetic biology
standard biological parts
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