Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina
Name
nihms-1519806.pdf
Description
Accepted version
Size
1.92 MB
Format
Adobe PDF
Checksum (MD5)
8e0e67dce20e6e43714fe787e625a913
Author(s)
Regev, Aviv
Date Issued
February 2019
Journal
Cell
Publisher
Elsevier BV
Citation
Peng, Yi-Rong et al. “Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina.” Cell 176 (2019): 1222-1237.e22 © 2019 The Author(s)
Version
Author's final manuscript
Abstract
High-acuity vision in primates, including humans, is mediated by a small central retinal region called the fovea. As more accessible organisms lack a fovea, its specialized function and its dysfunction in ocular diseases remain poorly understood. We used 165,000 single-cell RNA-seq profiles to generate comprehensive cellular taxonomies of macaque fovea and peripheral retina. More than 80% of >60 cell types match between the two regions but exhibit substantial differences in proportions and gene expression, some of which we relate to functional differences. Comparison of macaque retinal types with those of mice reveals that interneuron types are tightly conserved. In contrast, projection neuron types and programs diverge, despite exhibiting conserved transcription factor codes. Key macaque types are conserved in humans, allowing mapping of cell-type and region-specific expression of >190 genes associated with 7 human retinal diseases. Our work provides a framework for comparative single-cell analysis across tissue regions and species. Single-cell-based analysis provides a comprehensive molecular and cellular taxonomy of the primate retina.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.CELL.2019.01.004
