Validating Transcripts with Probes and Imaging Technology
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avano1.pdf
Description
Main Document
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522.72 KB
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e904cd474e0e2441335acc2ba0a54987
Author(s)
Itzkovitz, Shaul Shalev
van Oudenaarden, Alexander
Date Issued
March 2011
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Itzkovitz, Shalev, and Alexander van Oudenaarden. “Validating transcripts with probes and imaging technology.” Nature Methods 8.4s (2011): S12-S19.
Version
Author's final manuscript
Abstract
High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent technological advances in RNA fluorescence in situ hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled mRNA molecules of practically any endogenous gene. These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution.
MIT Department
MIT Materials Research Laboratory
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Physics
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nmeth.1573
