Development of an integrated capillary valve-based preconcentrator and surface-based immunoassay
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429466732-MIT.pdf
Description
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23.68 MB
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426b88def2891954e93a65f6364b4b50
Author(s)
Liu, Vincent Hok
Advisor(s)
Jongyoon Han.
Date Issued
2009
Publisher
Massachusetts Institute of Technology
Abstract
A new generation of integrated preconcentrator and immunoassay was developed. A novel, self-aligned method for patterning Nafion resin was developed and applied to create a preconcentrator. In a parallel effort, a surface-based immunoassay was developed and optimized for use with the preconcentrator. Experiments were carried out with R-phycoerythrin (R-PE) to characterize the performance of the integrated preconcentrator and immunoassay. The immunoassay originally has a detection limit of Ing/mL of R-PE in 1xPBS. When coupled to the preconcentrator, this limit has been improved to 0.Olng/mL, a roughly 100x improvement. Discussions of the differences between this and previous generations of concentrators are also presented in this thesis.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.
Includes bibliographical references (leaves 60-61).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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