Base extrusion is found at helical junctions between right- and left-handed forms of DNA and RNA
Name
Kim-2009-Base extrusion is found at helical junctions.pdf
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Author(s)
Kim, Doyoun
Reddy, Sanjith
Kim, Kong Young
Rich, Alexander
Lee, Sangho
Kim, Kyeong Kyu
Date Issued
May 2009
Journal
Nucleic Acids Research
Publisher
Oxford University Press
Citation
Kim, D. et al. “Base Extrusion Is Found at Helical Junctions Between Right- and Left-handed Forms of DNA and RNA.” Nucleic Acids Research 37.13 (2009): 4353–4359. Web.
Version
Final published version
Abstract
Base extrusion is a major structural feature at the junction between B- and Z-DNA (the B–Z junction) where a base pair is broken, and the two bases are extruded from the double helix. Despite the demonstration of base extrusion at the B–Z junction, it is not clear whether a similar base extrusion occurs at other types of junctions involving the left-handed Z conformation. Here, we investigate structural changes of bases at three Z-form junctions: DNA B–Z and Z–Z and RNA A–Z junctions. By monitoring fluorescently labeled duplex nucleic acids using 2-aminopurines at various positions relative to the junction point, we show that base extrusion occurs not only at the DNA B–Z junction, but also at the RNA A–Z and DNA Z–Z junctions. Our data suggest that base extrusion is a general feature of Z-form nucleic-acid junctions.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution Non-Commercial
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DOI of Published Version
https://doi.org/10.1093/nar/gkp364
