Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing
Name
nihms-1775391.pdf
Description
Accepted version
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1016.74 KB
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Checksum (MD5)
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Author(s)
Chattopadhyay, Sayani
Teixeira, Leandro BC
Kiessling, Laura L
McAnulty, Jonathan F
Raines, Ronald T
Date Issued
February 18, 2022
Journal
ACS Chemical Biology
Publisher
American Chemical Society (ACS)
Citation
Chattopadhyay, Sayani, Teixeira, Leandro BC, Kiessling, Laura L, McAnulty, Jonathan F and Raines, Ronald T. 2022. "Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing." ACS Chemical Biology, 17 (2).
Version
Author's final manuscript
Abstract
Transforming growth factor-β (TGF-β) plays important roles in wound healing. The activity of TGF-β is initiated upon the binding of the growth factor to the extracellular domains of its receptors. We sought to facilitate the activation by clustering these extracellular domains. To do so, we used a known peptide that binds to TGF-β receptors without diminishing their affinity for TGF-β. We conjugated this peptide to a collagen-mimetic peptide that can anneal to the damaged collagen in a wound bed. We find that the conjugate enhances collagen deposition and wound closure in mice in a manner consistent with the clustering of TGF-β receptors. This strategy provides a means to upregulate the TGF-β signaling pathway without adding exogenous TGF-β and could inspire means to treat severe wounds.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1021/acschembio.1c00745
