Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
Name
Chan-2013-Heterologous Stop Co.pdf
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373.13 KB
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Author(s)
Jungreis, Irwin
Kellis, Manolis
Chan, Clara Sophia
Date Issued
March 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Chan, Clara S., Irwin Jungreis, and Manolis Kellis 2013Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast. Joseph Schacherer, ed. PLoS ONE 8(3): e59450.
Version
Final published version
Abstract
Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Mathematics
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0059450
