An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
Name
Chahal-2017-An RNA nanoparticle vaccine agains.pdf
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1.39 MB
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Author(s)
Woodham, Andrew W.
Chahal, Jasdave
Fang, Taotao
Khan, Omar Fizal
Ling, Jingjing
Anderson, Daniel Griffith
Ploegh, Hidde
Date Issued
March 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Chahal, Jasdave S.; Fang, Tao; Woodham, Andrew W.; Khan, Omar F.; Ling, Jingjing; Anderson, Daniel G. and Ploegh, Hidde L. “An RNA Nanoparticle Vaccine Against Zika Virus Elicits Antibody and CD8⁺ T Cell Responses in a Mouse Model.” Scientific Reports 7, no. 1 (March 2017): 252 © 2017 The Author(s)
Version
Final published version
Abstract
The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/6 mice as assayed by ELISA. Using this tool, we identified a unique H-2D[superscript b]-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.
MIT Department
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/s41598-017-00193-w
