Hedgehog signaling regulates gene expression in planarian glia
Name
Wang-2016-Hedgehog signaling r.pdf
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44.57 MB
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Author(s)
Scimone, M Lucila
Clandinin, Thomas R
Wang, Irving E.
Reddien, Peter
Lapan, Sylvain W.
Date Issued
September 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Wang, Irving E., Sylvain W. Lapan, M. Lucila Scimone, Thomas R. Clandinin, and Peter W. Reddien. “Hedgehog Signaling Regulates Gene Expression in Planarian Glia.” eLife 5 (September 9, 2016). p.1-26.
Version
Final published version
Abstract
Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh[superscript +] neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.7554/eLife.16996
