High-Bandwidth AFM-Based Rheology Reveals that Cartilage is Most Sensitive to High Loading Rates at Early Stages of Impairment
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Author(s)
Tavakoli Nia, Hadi
Soltani Bozchalooi, Iman
Li, Yang
Han, Lin
Hung, Han-Hwa K.
Frank, Eliot
Youcef-Toumi, Kamal
Ortiz, Christine
Grodzinsky, Alan J.
Date Issued
April 2013
Journal
Biophysical Journal
Publisher
Elsevier B.V.
Citation
Nia, Hadi Tavakoli, Iman S. Bozchalooi, Yang Li, Lin Han, Han-Hwa Hung, Eliot Frank, Kamal Youcef-Toumi, Christine Ortiz, and Alan Grodzinsky. “High-Bandwidth AFM-Based Rheology Reveals That Cartilage Is Most Sensitive to High Loading Rates at Early Stages of Impairment.” Biophysical Journal 104, no. 7 (April 2013): 1529–1537.
Version
Final published version
Abstract
Utilizing a newly developed atomic-force-microscopy-based wide-frequency rheology system, we measured the dynamic nanomechanical behavior of normal and glycosaminoglycan (GAG)-depleted cartilage, the latter representing matrix degradation that occurs at the earliest stages of osteoarthritis. We observed unique variations in the frequency-dependent stiffness and hydraulic permeability of cartilage in the 1 Hz-to-10 kHz range, a frequency range that is relevant to joint motions from normal ambulation to high-frequency impact loading. Measurement in this frequency range is well beyond the capabilities of typical commercial atomic force microscopes. We showed that the dynamic modulus of cartilage undergoes a dramatic alteration after GAG loss, even with the collagen network still intact: whereas the magnitude of the dynamic modulus decreased two- to threefold at higher frequencies, the peak frequency of the phase angle of the modulus (representing fluid-solid frictional dissipation) increased 15-fold from 55 Hz in normal cartilage to 800 Hz after GAG depletion. These results, based on a fibril-reinforced poroelastic finite-element model, demonstrated that GAG loss caused a dramatic increase in cartilage hydraulic permeability (up to 25-fold), suggesting that early osteoarthritic cartilage is more vulnerable to higher loading rates than to the conventionally studied “loading magnitude”. Thus, over the wide frequency range of joint motion during daily activities, hydraulic permeability appears the most sensitive marker of early tissue degradation.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2013.02.048
