Ru-Porphyrin Protein Scaffolds for Sensing O[subscript 2]
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Nocera_Ru-porphyrin protein.pdf
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Author(s)
Winter, Michael B.
McLaurin, Emily J.
Reece, Steven Y.
Olea, Charles
Nocera, Daniel G.
Marletta, Michael A.
Alternative Title
Ru-Porphyrin Protein Scaffolds for Sensing O2
Date Issued
April 2010
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Winter, Michael B., Emily J. McLaurin, Steven Y. Reece, Charles Olea, Daniel G. Nocera, and Michael A. Marletta. “Ru-Porphyrin Protein Scaffolds for Sensing O[subscript 2].” Journal of the American Chemical Society 132, no. 16 (April 28, 2010): 5582-5583.
Version
Author's final manuscript
Abstract
Hemoprotein-based scaffolds containing phosphorescent ruthenium(II) CO mesoporphyrin IX (RuMP) are reported here for oxygen (O2) sensing in biological contexts. RuMP was incorporated into the protein scaffolds during protein expression utilizing a novel method that we have described previously. A high-resolution (2.00 Å) crystal structure revealed that the unnatural porphyrin binds to the proteins in a manner similar to the native heme and does not perturb the protein fold. The protein scaffolds were found to provide unique coordination environments for RuMP and modulate the porphyrin emission properties. Emission lifetime measurements demonstrate a linear O2 response within the physiological range and precision comparable to commercial O2 sensors. The RuMP proteins are robust, readily modifiable platforms and display promising O2 sensing properties for future in vivo applications.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ja101527r
