Expansion microscopy: enabling single cell analysis in intact biological systems
Name
nihms977451.pdf
Description
Accepted version
Size
1.52 MB
Format
Adobe PDF
Checksum (MD5)
c89ec1d2c5c870d47010ccf4e3b8d1df
Author(s)
Alon, Shahar
Huynh, Grace H.
Boyden, Edward S.
Date Issued
April 2019
Journal
FEBS journal
Publisher
Wiley
Citation
Alon, Shahar, Grace H. Huynh, and Edward S. Boyden, "Expansion microscopy: enabling single cell analysis in intact biological systems." FEBS journal 286, 8 (April 2019): p. 1482-94 doi 10.1111/febs.14597 2019 ©2019 Author(s)
Version
Author's final manuscript
Abstract
There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecules throughout cells and intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact systems. Expansion microscopy (ExM) is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution microscopy on diffraction-limited microscopes, enabling rapid image acquisition and large field of view. As a result, ExM is well-positioned to integrate molecular content and cellular morphology, with the spatial precision sufficient to resolve individual biological building blocks, and the scale and accessibility required to deploy over extended 3-D objects like tissues and organs. ©2019
MIT Department
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1111/febs.14597
