Expansion microscopy: principles and uses in biological research
Name
nihms-1010719.pdf
Description
Accepted version
Size
1014.7 KB
Format
Adobe PDF
Checksum (MD5)
d90f66fdcb51f16d4758e37acdd104ea
Author(s)
Wassie, Asmamaw T.
Zhao, Yongxin
Boyden, Edward
Date Issued
December 2018
Journal
Nature Methods
Publisher
Springer Nature
Citation
Wassie, Asmamaw T. et al., "Expansion Microscopy: Principles and Uses in Biological Research." Nature Methods 16, 1 (January 2019): 33–41 @2018 Authors
Version
Author's final manuscript
Abstract
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Media Laboratory
Massachusetts Institute of Technology. Center for Neurobiological Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41592-018-0219-4
