Synthetic Developmental Biology: Understanding Through Reconstitution
Name
nihms-1694059.pdf
Description
Accepted version
Size
1.55 MB
Format
Adobe PDF
Checksum (MD5)
d846da3de35fe820d5e652fe39e48fe3
Author(s)
Schlissel, Gavin
Li, Pulin
Date Issued
2020
Journal
Annual Review of Cell and Developmental Biology
Publisher
Annual Reviews
Version
Author's final manuscript
Abstract
© 2020 Annual Reviews Inc.. All rights reserved. Reconstitution is an experimental strategy that seeks to recapitulate biological events outside their natural contexts using a reduced set of components. Classically, biochemical reconstitution has been extensively applied to identify the minimal set of molecules sufficient for recreating the basic chemistry of life. By analogy, reconstitution approaches to developmental biology recapitulate aspects of developmental events outside an embryo, with the goal of revealing the basic genetic circuits or physical cues sufficient for recreating developmental decisions. The rapidly growing repertoire of genetic, molecular, microscopic, and bioengineering tools is expanding the complexity and precision of reconstitution experiments. We review the emerging field of synthetic developmental biology, with a focus on the ways in which reconstitution strategies and new biological tools have enhanced our modern understanding of fundamental questions in developmental biology.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1146/ANNUREV-CELLBIO-020620-090650
