Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes
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Regev_Early-branching.pdf
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Author(s)
Solomon, Kevin V.
Haitjema, Charles H.
Henske, John K.
Gilmore, Sean P.
Lipzen, Anna
Brewer, Heather M.
Purvine, Samuel O.
Wright, Aaron T.
Theodorou, Michael K.
Grigoriev, Igor V.
Date Issued
March 2016
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Solomon, K. V., C. H. Haitjema, J. K. Henske, S. P. Gilmore, D. Borges-Rivera, A. Lipzen, H. M. Brewer, et al. “Early-Branching Gut Fungi Possess a Large, Comprehensive Array of Biomass-Degrading Enzymes.” Science 351, no. 6278 (February 18, 2016): 1192–1195.
Version
Author's final manuscript
Abstract
The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing.
Description
available in PMC 2016 November 07
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1126/science.aad1431
