Label-free volumetric optical imaging of intact murine brains
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Label-free volumetric.pdf
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Author(s)
Ren, Jianbiao
Choi, Heejin
Chung, Kwanghun
Bouma, Brett E.
Date Issued
April 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Ren, Jian, Heejin Choi, Kwanghun Chung, and Brett E. Bouma. “Label-Free Volumetric Optical Imaging of Intact Murine Brains.” Scientific Reports 7 (April 12, 2017): 46306.
Version
Final published version
Abstract
A central effort of today’s neuroscience is to study the brain’s ’wiring diagram’. The nervous system is believed to be a network of neurons interacting with each other through synaptic connection between axons and dendrites, therefore the neuronal connectivity map not only depicts the underlying anatomy, but also has important behavioral implications. Different approaches have been utilized to decipher neuronal circuits, including electron microscopy (EM) and light microscopy (LM). However, these approaches typically demand extensive sectioning and reconstruction for a brain sample. Recently, tissue clearing methods have enabled the investigation of a fully assembled biological system with greatly improved light penetration. Yet, most of these implementations, still require either genetic or exogenous contrast labeling for light microscopy. Here we demonstrate a high-speed approach, termed as Clearing Assisted Scattering Tomography (CAST), where intact brains can be imaged at optical resolution without labeling by leveraging tissue clearing and the scattering contrast of optical frequency domain imaging (OFDI).
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/srep46306
