Cytosolic Delivery of Functional Ubiquitin
Name
Journal of Peptide Science - 2025 - Giancola - Cytosolic Delivery of Functional Ubiquitin.pdf
Description
Published version
Size
1018.73 KB
Format
Adobe PDF
Checksum (MD5)
17b89b0c304f7c7b802af8b93f5ec2d5
Author(s)
Giancola, JoLynn B
Okon, Aniekan
Li, Yanfeng
Strieter, Eric R
Raines, Ronald T
Date Issued
May 8, 2025
Journal
Journal of Peptide Science
Publisher
Wiley
Citation
Giancola, J., Okon, A., Li, Y., Strieter, E. and Raines, R. (2025), Cytosolic Delivery of Functional Ubiquitin. J Pept Sci, 31: e70026.
Version
Final published version
Abstract
The proteostasis network involves complex protein signaling cascades. The tagging of proteins with ubiquitin is central to thedegradation of cellular proteins, but understanding its exact role in processing proteins is complicated by the complexity andextent of its utilization within cells. Here, we describe the application of a traceless protein delivery strategy to effect the uptakeof exogenous ubiquitin into the cytosol of human cells. We find that coadministration of the endosomolytic peptides L17E and,especially, L17ER 4 provides not only cytosolic access to ubiquitin but also its functional incorporation into endogenous proteins.By enabling the study of semisynthetic ubiquitin variants in the human cytosol, this strategy could advance the field of ubiquitinbiology.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/psc.70026
