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Temporally precise single-cell-resolution optogenetics
Name
nihms909827.pdf
Description
Accepted version
Size
2.39 MB
Format
Adobe PDF
Checksum (MD5)
5f1896a4d22299c50d48d21bfb02d06d
Author(s)
Shemesh, Or A
Tanese, Dimitrii
Zampini, Valeria
Linghu, Changyang
Piatkevich, Kiryl
Ronzitti, Emiliano
Papagiakoumou, Eirini
Boyden, Edward S
Emiliani, Valentina
Date Issued
2017
Journal
Nature Neuroscience
Publisher
Springer Nature
Version
Author's final manuscript
Abstract
© 2017 The Author(s). Optogenetic control of individual neurons with high temporal precision within intact mammalian brain circuitry would enable powerful explorations of how neural circuits operate. Two-photon computer-generated holography enables precise sculpting of light and could in principle enable simultaneous illumination of many neurons in a network, with the requisite temporal precision to simulate accurate neural codes. We designed a high-efficacy soma-targeted opsin, finding that fusing the N-terminal 150 residues of kainate receptor subunit 2 (KA2) to the recently discovered high-photocurrent channelrhodopsin CoChR restricted expression of this opsin primarily to the cell body of mammalian cortical neurons. In combination with two-photon holographic stimulation, we found that this somatic CoChR (soCoChR) enabled photostimulation of individual cells in mouse cortical brain slices with single-cell resolution and <1-ms temporal precision. We used soCoChR to perform connectivity mapping on intact cortical circuits.
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
10.1038/S41593-017-0018-8
