Dual Molecular Signals Mediate the Bacterial Response to Outer-Membrane Stress
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Sauer_Dual molecular.pdf
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Author(s)
Lima, Santiago
Guo, Monica S.
Chaba, Rachna
Gross, Carol A.
Sauer, Robert T
Date Issued
May 2013
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Lima, Santiago, Monica S. Guo, Rachna Chaba, Carol A. Gross, and Robert T. Sauer. “Dual Molecular Signals Mediate the Bacterial Response to Outer-Membrane Stress.” Science 340, no. 6134 (May 16, 2013): 837–841.
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Author's final manuscript
Abstract
In Gram-negative bacteria, outer-membrane integrity is essential for survival and is monitored by the σ[superscript E] stress-response system, which initiates damage-repair pathways. One activating signal is unassembled outer-membrane proteins. Using biochemical and genetic experiments in Escherichia coli, we found that off-pathway intermediates in lipopolysaccharide transport and assembly provided an additional required signal. These distinct signals, arising from disruptions in the transport and assembly of the major outer-membrane components, jointly determined the rate of proteolytic destruction of a negative regulator of the σ[superscript E] transcription factor, thereby modulating the expression of stress-response genes. This dual-signal system permits a rapid response to dysfunction in outer-membrane biogenesis, while buffering responses to transient fluctuations in individual components, and may represent a broad strategy for bacteria to monitor their interface with the environment.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1235358
