Helicobacter Species Identified in Captive Sooty Mangabeys (Cercocebus atys) with Metastatic Gastric Adenocarcinoma
Name
nihms764514.pdf
Size
1.07 MB
Format
Adobe PDF
Checksum (MD5)
53152ab1674cbeb54ae84f5e9502c988
Author(s)
Esmail, Michael Y.
Bacon, Rebecca
Swennes, Alton G.
Feng, Yan
Shen, Zeli
Garcia, AnaPatricia
Sharma, Prachi
Cohen, Joyce
Fox, James G.
Date Issued
June 2016
Journal
Helicobacter
Publisher
Wiley
Citation
Esmail, Michael Y., et al., "Helicobacter Species Identified in Captive Sooty Mangabeys (Cercocebus atys) with Metastatic Gastric Adenocarcinoma." Helicobacter 21, 3 (June 2016): p. 175-85 doi 10.1111/HEL.12269 ©2016 Author(s)
Version
Author's final manuscript
Abstract
Background: Of all human cancers, gastric carcinoma is the one of the leading causes of death. Helicobacter pylori is considered a major etiologic agent of this disease. Spontaneously occurring gastric carcinoma is a rare diagnosis in nonhuman primates. A 2011 case report documented a high incidence of gastric adenocarcinoma in a closed colony of captive sooty mangabeys (Cercebus atys). However, H. pylori infection was not detected in these animals. Materials and Methods: In this study, using archived formalin-fixed, paraffin-embedded stomach sections of these animals alternative methodologies were used to identify H. pylori and other non-H. pylori Helicobacter species. In addition, two additional cases of sooty mangabeys with metastatic gastric carcinoma are characterized. Results: Using fluorescent in situ hybridization, we identified gastric H. suis in 75% of archived and new gastric carcinoma cases. In the two newly reported cases, H. suis and a novel Helicobacter species were detected via PCR and sequence analysis of the 16S rRNA gene. H. pylori was not identified in any of the gastric carcinoma cases via FISH and/or PCR and sequence analysis of Helicobacter spp. in DNA from of available tissues. Conclusions: This report is the first to characterize Helicobacter species infection in spontaneous gastric carcinoma with metastatic potential in nonhuman primates. ©2016
MIT Department
Massachusetts Institute of Technology. Division of Comparative Medicine
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1111/HEL.12269
