Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus
Name
1-s2.0-S2589004218300750-main.pdf
Description
Published version
Size
4.11 MB
Format
Adobe PDF
Checksum (MD5)
f1be5338c04116f9f9ae3257c4bd95d4
Author(s)
Laub, Michael T
Date Issued
June 2018
Journal
iScience
Publisher
Elsevier BV
Citation
Lambert, Ambroise et al. “Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus.” iScience 4 (2018): 180-189 © 2018 The Author(s)
Version
Final published version
Abstract
Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral wallsand then via a combination of zonal elongation and constriction at the division site to form the poles ofdaughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size con-trol and homeostasis has rarely been considered. To reveal the roles of cell elongation and constrictionin bacterial size regulation during cell division, we captured the shape dynamics ofCaulobactercres-centuswith time-lapse structured illumination microscopy and used molecular markers as cell-cyclelandmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constriction rate contrib-utes to both size control and homeostasis, by determining elongation during constriction and bycompensating for variation in pre-constriction elongation on a single-cell basis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.ISCI.2018.05.020
