TALEN-mediated editing of the mouse Y chromosome
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Jaenisch_TALEN-mediated.pdf
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1.28 MB
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Checksum (MD5)
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Author(s)
Wang, Haoyi
Hu, Yueh-Chiang
Markoulaki, Styliani
Welstead, G Grant
Shivalila, Chikdu Shakti
Pyntikova, Tatyana
Dadon, Daniel Benjamin
Voytas, Daniel F.
Bogdanove, Adam J.
Jaenisch, Rudolf
Date Issued
May 2013
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Wang, Haoyi, Yueh-Chiang Hu, Styliani Markoulaki, G Grant Welstead, Albert W Cheng, Chikdu S Shivalila, Tatyana Pyntikova, et al. “TALEN-Mediated Editing of the Mouse Y Chromosome.” Nature Biotechnology 31, no. 6 (May 12, 2013): 530–532.
Version
Author's final manuscript
Abstract
The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes—Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
MIT Department
Massachusetts Institute of Technology. Computational and Systems Biology Program
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nbt.2595
