Portable, On-Demand Biomolecular Manufacturing
Name
Portable on-Demand Biomolecular Manufacturing-Collins.pdf
Size
1.62 MB
Format
Adobe PDF
Checksum (MD5)
6aeb4394b2aab75fc400c169b2e5c16f
Author(s)
Pardee, Keith
Nguyen, Peter Q.
Donghia, Nina
Burrill, Devin
Ferrante, Tom
McSorley, Fern R.
Furuta, Yoshikazu
Vernet, Andyna
Lewandowski, Michael
Boddy, Christopher N.
Date Issued
September 2016
Journal
Cell
Publisher
Elsevier
Citation
Pardee, Keith et al. “Portable, On-Demand Biomolecular Manufacturing.” Cell 167, 1 (September 2016): 248–259 © 2016 Elsevier
Version
Author's final manuscript
Abstract
Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs, therapeutic proteins, and other commodities. However, the need for specialized equipment and refrigeration for production and distribution poses a challenge for the delivery of these technologies to the field and to low-resource areas. Here, we present a portable platform that provides the means for on-site, on-demand manufacturing of therapeutics and biomolecules. This flexible system is based on reaction pellets composed of freeze-dried, cell-free transcription and translation machinery, which can be easily hydrated and utilized for biosynthesis through the addition of DNA encoding the desired output. We demonstrate this approach with the manufacture and functional validation of antimicrobial peptides and vaccines and present combinatorial methods for the production of antibody conjugates and small molecules. This synthetic biology platform resolves important practical limitations in the production and distribution of therapeutics and molecular tools, both to the developed and developing world.
MIT Department
Institute for Medical Engineering and Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.cell.2016.09.013
