Genetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation
Name
Advanced Materials - 2025 - Sedenho - Genetic Surfaceome E coli Reprogramming Enables Selective Water Oxidation.pdf
Description
Published version
Size
1.98 MB
Format
Adobe PDF
Checksum (MD5)
d019262817a7d35b160f9c3df8a9d7a1
Author(s)
Sedenho, Graziela C
Pacheco, Jéssica C
Gut, Melanie
Lima, Filipe CDA
Dey, Sunanda
Crespilho, Frank N
Furst, Ariel L
Date Issued
August 15, 2025
Journal
Advanced Materials
Publisher
Wiley
Citation
G. C. Sedenho, J. C. Pacheco, M. Gut, et al. “ Genetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation.” Adv. Mater. (2025): e08100.
Version
Final published version
Abstract
Programming catalytic behavior at the microbial genome level is a frontier in synthetic biology with direct impact on bioelectrocatalysis. A key challenge is the coordinated control of gene expression, localization, folding, and cofactor maturation required to achieve proper bioelectrocatalytic activity. Here, a synthetic operon in Escherichia coli is engineered to reprogram its surfaceome for selective water oxidation. Using orthogonal IPTG-inducible control and codon-optimized expression, a fungal bilirubin oxidase (BOD) displayed at the cell surface is produced by ice nucleation protein anchoring (BOD-E. coli). Post-overexpression copper catalytic site reconstitution provides an active holoenzyme. The developed engineered living material performs water oxidation at near-zero overpotential (27 mV at pH 9.1), with complete suppression of the oxygen reduction reaction. These results show how regenerable microbial platforms can be designed for selective catalysis and artificial photosynthesis.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/adma.202508100
