Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry
Name
s41467-021-23416-1.pdf
Description
Published version
Size
4.42 MB
Format
Unknown
Checksum (MD5)
1d64374aa6227aa6f04bba2362c69c92
Author(s)
Cho, Soo-Yeon
Gong, Xun
Koman, Volodymyr B.
Kuehne, Matthias
Moon, Sun Jin
Son, Manki
Lew, Tedrick Thomas Salim
Gordiichuk, Pavlo
Jin, Xiaojia
Sikes, Hadley D.
Date Issued
May 2021
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Version
Final published version
Abstract
AbstractNanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41467-021-23416-1
