Genome-wide genetic screening in the mammalian CNS
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Wertz-Heiman2017_Chapter_Genome-WideGeneticScreeningInT.pdf
Description
Published version
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222.66 KB
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Adobe PDF
Checksum (MD5)
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Author(s)
Wertz, Mary H.
Heiman, Myriam
Date Issued
2017
Journal
Genome editing in neurosciences
Publisher
Springer International Publishing
Citation
Wertz, Mary H., and Heiman, Myriam, "Genome-wide genetic screening in the mammalian CNS." In Jaenisch, Rudolf, Feng Zhang, and Fred Gage, eds., Genome editing in neurosciences (Cham: Springer, 2017): p. 31-39 doi 10.1007/978-3-319-60192-2_3 ©2017 Author(s)
Version
Final published version
Abstract
Genes linked to major neurodegenerative diseases, including Alzheimer’s, Parkinson’s, and Huntington’s diseases, were first identified over 15 years ago, but neither a full molecular explanation for the cell loss seen in human patients nor a curative therapy has yet been achieved for any of these diseases. In most model organisms, when new hypotheses are needed to explain a cellular process, genetic screens are the tool of choice. For example, ‘synthetic lethal’ screens can lead to the identification of genes that enhance the toxicity of a particular mutation, revealing pathways critical for surviving the mutation’s effects. To date, however, genome-wide unbiased screens are not feasible in mammalian central nervous system neurons except in vitro, which fails to capture the relevant disease pathologies, and no genome-wide screens have yet been conducted in the mammalian central nervous system. We outline in this short monograph the steps needed to implement a methodology that allows for genome-wide genetic screening in the central nervous system of mice to study both normal and degenerative disease gene function. ©2017
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1007/978-3-319-60192-2_3
