The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant
Name
Wei-2014-The class III ribonu.pdf
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Author(s)
Wei, Yifeng
Funk, Michael Andrew
Rosado, Leonardo A.
Baek, Jiyeon
Stubbe, JoAnne
Drennan, Catherine L
Date Issued
August 2014
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Wei, Y., M. A. Funk, L. A. Rosado, J. Baek, C. L. Drennan, and J. Stubbe. “The Class III Ribonucleotide Reductase from Neisseria Bacilliformis Can Utilize Thioredoxin as a Reductant.” Proceedings of the National Academy of Sciences 111, no. 36 (August 25, 2014): E3756–E3765.
Version
Final published version
Abstract
The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO[subscript 2]. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architecture and the position of conserved residues in the active site. Phylogenetic studies suggest that this form of class III RNR is present in bacteria and archaea that carry out diverse types of anaerobic metabolism.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
https://doi.org/10.1073/pnas.1414396111
