Novel Normalization Standard using Fluorescence
Name
BBFRFC78.pdf
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80.28 KB
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Author(s)
Bukhari, Syed Habib Tahir
Akkineni, Ashwini Rahul
Ananth, Adithya
Gordeev, Victor
Grieb, Svea
Mansour, Sarah
Roth, Mareike
Sampathkumar, Charanya
Schirmer, Lucas
Tam, Jonathan
Date Issued
December 5, 2010
Series/Report no.
BBF RFC;78
Abstract
The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, an inducible promoter drives the expression of the second reporter, YFP in the case of the part tested. The constitutively expressed reporter protein (R1) serves as normalization factor for the inducible reporter (R2) by simple division.
Subjects
measurement standard
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Attribution-No Derivative Works 3.0 United States
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