Experimental estimate of the diffusivity of Vascular Endothelial Growth Factor
Name
77561721-MIT.pdf
Description
Full printable version
Size
821.42 KB
Format
Adobe PDF
Checksum (MD5)
c90183149177150d5f8b63e887ddeaa5
Author(s)
Núñez, Daniel A
Advisor(s)
Roger D. Kamm.
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
The diffusivity of Vascular Endothelial Growth Factor (VEGF) is a number that is very important in determining the transport of VEGF. The transport of VEGF determines crucial processes such as angiogenesis and vasculogenesis. This study aimed at obtaining an estimate of the diffusivity of VEGF by using a simple system consisting of an insert with a collagen membrane placed within the well of a cell culture plate. A solution of VEGF was added to the insert, and the same solution without VEGF was added to the well surrounding the insert. The VEGF diffusion was monitored by taking samples in time of both the upstream and downstream baths, and analyzing the samples with an ELISA. Modeling the diffusion as one-dimensional, it was possible to estimate the diffusivity from the change in downstream concentration with time. The diffusivity was estimated to be around 5.55e⁻⁷ cm²sec.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.
Includes bibliographical references (leaf 16).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
Persistent DSpace Link
