Receptor specificity does not affect replication or virulence of the 2009 pandemic H1N1 influenza virus in mice and ferrets
Name
Receptor Specificity.pdf
Size
1.44 MB
Format
Adobe PDF
Checksum (MD5)
0db622b2046f1d62dc0bed14af6e190b
Author(s)
Lakdawala, Seema S.
Shih, Angela R.
Jayaraman, Akila
Lamirande, Elaine W.
Moore, Ian
Paskel, Myeisha
Sasisekharan, Ram
Subbarao, Kanta
Date Issued
September 2013
Journal
Virology
Publisher
Elsevier
Citation
Lakdawala, Seema S., Angela R. Shih, Akila Jayaraman, Elaine W. Lamirande, Ian Moore, Myeisha Paskel, Ram Sasisekharan, and Kanta Subbarao. “Receptor Specificity Does Not Affect Replication or Virulence of the 2009 Pandemic H1N1 Influenza Virus in Mice and Ferrets.” Virology 446, no. 1–2 (November 2013): 349–356.
Version
Author's final manuscript
Abstract
Human influenza viruses predominantly bind α2,6 linked sialic acid (SA) while avian viruses bind α2,3 SA-containing complex glycans. Virulence and tissue tropism of influenza viruses have been ascribed to this binding preference. We generated 2009 pandemic H1N1 (pH1N1) viruses with either predominant α2,3 or α2,6 SA binding and evaluated these viruses in mice and ferrets. The α2,3 pH1N1 virus had similar virulence in mice and replicated to similar titers in the respiratory tract of mice and ferrets as the α2,6 and WT pH1N1 viruses. Immunohistochemical analysis determined that all viruses infected similar cell types in ferret lungs. There is increasing evidence that receptor specificity of influenza viruses is more complex than the binary model of α2,6 and α2,3 SA binding and our data suggest that influenza viruses use a wide range of SA moieties to infect host cells.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.virol.2013.08.011
