In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy
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PUB128-APL1052014-ZhuoS.pdf
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Checksum (MD5)
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Author(s)
Zhuo, Shuangmu
Yan, Jie
Kang, Yuzhan
Xu, Shuoyu
Peng, Qiwen
So, Peter T. C.
Yu, Hanry
Date Issued
July 2014
Journal
Applied Physics Letters
Publisher
AIP Publishing
Citation
Zhuo, Shuangmu, Jie Yan, Yuzhan Kang, Shuoyu Xu, Qiwen Peng, Peter T. C. So, and Hanry Yu. “In Vivo, Label-Free, Three-Dimensional Quantitative Imaging of Liver Surface Using Multi-Photon Microscopy.” Applied Physics Letters 105, no. 2 (July 14, 2014): 023701. © 2014 AIP Publishing LLC
Version
Final published version
Abstract
Various structural features on the liver surface reflect functional changes in the liver. The visualization of these surface features with molecular specificity is of particular relevance to understanding the physiology and diseases of the liver. Using multi-photon microscopy (MPM), we have developed a label-free, three-dimensional quantitative and sensitive method to visualize various structural features of liver surface in living rat. MPM could quantitatively image the microstructural features of liver surface with respect to the sinuosity of collagen fiber, the elastic fiber structure, the ratio between elastin and collagen, collagen content, and the metabolic state of the hepatocytes that are correlative with the pathophysiologically induced changes in the regions of interest. This study highlights the potential of this technique as a useful tool for pathophysiological studies and possible diagnosis of the liver diseases with further development. © 2014 AIP Publishing LLC.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1063/1.4890593
