Reprogramming of Human Peripheral Blood Cells to Induced Pluripotent Stem Cells
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Staerk-2010-Reprogramming of Hum.pdf
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Author(s)
Staerk, Judith
Dawlaty, Meelad M.
Gao, Qing
Maetzel, Dorothea
Hanna, Jacob
Sommer, Cesar A.
Mostoslavsky, Gustavo
Jaenisch, Rudolf
Date Issued
July 2010
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
Staerk, Judith, Meelad M. Dawlaty, Qing Gao, Dorothea Maetzel, Jacob Hanna, Cesar A. Sommer, Gustavo Mostoslavsky, and Rudolf Jaenisch. “Reprogramming of Human Peripheral Blood Cells to Induced Pluripotent Stem Cells.” Cell Stem Cell 7, no. 1 (July 2010): 20–24. © 2010 Elsevier Inc.
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Final published version
Abstract
Embryonic stem cells are pluripotent cells derived from the inner cell mass of the developing embryo that have the capacity to differentiate into every cell type of the adult (Evans and Kaufman, 1981, Martin, 1981, Martin and Evans, 1975 and Thomson et al., 1998). The generation of patient-specific pluripotent cells is therefore an important goal of regenerative medicine. A major step to achieve this was the recent discovery that ectopic expression of defined transcription factors induces pluripotency in somatic cells (Lowry et al., 2008, Park et al., 2008b, Takahashi et al., 2007 and Yu et al., 2007). Until now, the most common source from which to derive human iPSCs has been skin fibroblasts (Lowry et al., 2008, Park et al., 2008a, Park et al., 2008b, Takahashi et al., 2007 and Yu et al., 2009). However, the requirement for skin biopsies and the need to expand fibroblast cells for several passages in vitro represent a hurdle that must be overcome to make iPSC technology broadly applicable. Peripheral blood can be utilized as an easily accessible source of patient tissue for reprogramming. Here we derived iPSCs from frozen human peripheral blood samples. Some of the iPSCs had rearrangements of the T cell receptor (TCR), indicating that T cells can be reprogrammed to pluripotency.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1016/j.stem.2010.06.002
