Cytotoxic Escherichia coli strains encoding colibactin colonize laboratory mice
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Author(s)
Garcia, Alexis
Mannion, Anthony
Feng, Yan
Madden, Carolyn
Bakthavatchalu, Vasudevan
Shen, Zeli
Ge, Zhongming
Fox, James G
Date Issued
July 2016
Journal
Microbes and Infection
Publisher
Elsevier
Citation
García, Alexis et al. “Cytotoxic Escherichia Coli Strains Encoding Colibactin Colonize Laboratory Mice.” Microbes and Infection 18, 12 (December 2016): 777–786 © 2016 Institut Pasteur
Version
Author's final manuscript
Abstract
Escherichia coli strains have not been fully characterized in laboratory mice and are not currently excluded from mouse colonies. Colibactin (Clb), a cytotoxin, has been associated with inflammation and cancer in humans and animals. We performed bacterial cultures utilizing rectal swab, fecal, and extra intestinal samples from clinically unaffected or affected laboratory mice. Fifty-one E. coli were isolated from 45 laboratory mice, identified biochemically, and selected isolates were serotyped. The 16S rRNA gene was amplified and sequenced for specific isolates, PCR used for clbA and clbQ gene amplification, and phylogenetic group identification was performed on all 51 E. coli strains. Clb genes were sequenced and selected E. coli isolates were characterized using a HeLa cell cytotoxicity assay. Forty-five of the 51 E. coli isolates (88%) encoded clbA and clbQ and belonged to phylogenetic group B2. Mouse E. coli serotypes included: O2:H6, O−:H−, OM:H+, and O22:H−. Clb-encoding O2: H6 mouse E. coli isolates were cytotoxic in vitro. A Clb-encoding E. coli was isolated from a clinically affected genetically modified mouse with cystic endometrial hyperplasia. Our findings suggest that Clb-encoding E. coli colonize laboratory mice and may induce clinical and subclinical diseases that may impact experimental mouse models. Keywords: Escherichia coli; Colibactin; Mice; Disease; Cytotoxic; Cancer
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.MICINF.2016.07.005
