Intercellular trafficking of the nuclear oncoprotein DEK
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Ploegh_Intercellular trafficking.pdf
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1.5 MB
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Author(s)
Ploegh, Hidde
Saha, Anjan K.
Kappes, Ferdinand
Mundade, Amruta
Deutzmann, Anja
Rosmarin, David M.
Legendre, Maureen
Chatain, Nicolas
Al-Obaidi, Zeina
Adams, Barbara S.
Date Issued
April 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Saha, A. K., F. Kappes, A. Mundade, A. Deutzmann, D. M. Rosmarin, M. Legendre, N. Chatain, et al. “Intercellular trafficking of the nuclear oncoprotein DEK.” Proceedings of the National Academy of Sciences 110, no. 17 (April 23, 2013): 6847-6852.
Version
Final published version
Abstract
DEK is a biochemically distinct, conserved nonhistone protein that is vital to global heterochromatin integrity. In addition, DEK can be secreted and function as a chemotactic, proinflammatory factor. Here we show that exogenous DEK can penetrate cells, translocate to the nucleus, and there carry out its endogenous nuclear functions. Strikingly, adjacent cells can take up DEK secreted from synovial macrophages. DEK internalization is a heparan sulfate-dependent process, and cellular uptake of DEK into DEK knockdown cells corrects global heterochromatin depletion and DNA repair deficits, the phenotypic aberrations characteristic of these cells. These findings thus unify the extracellular and intracellular activities of DEK, and suggest that this paracrine loop involving DEK plays a role in chromatin biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1220751110
