Principles for the design of multicellular engineered living systems
Name
5.0076635.pdf
Description
Published version
Size
13.86 MB
Format
Adobe PDF
Checksum (MD5)
91005c3539f6d7efe4b6f6807d7b6c8a
Author(s)
Aydin, Onur
Passaro, Austin P
Raman, Ritu
Spellicy, Samantha E
Weinberg, Robert P
Kamm, Roger D
Sample, Matthew
Truskey, George A
Zartman, Jeremiah
Dar, Roy D
Date Issued
2022
Journal
APL Bioengineering
Publisher
AIP Publishing
Citation
Aydin, Onur, Passaro, Austin P, Raman, Ritu, Spellicy, Samantha E, Weinberg, Robert P et al. 2022. "Principles for the design of multicellular engineered living systems." APL Bioengineering, 6 (1).
Version
Final published version
Abstract
Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell–cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the “black box” of living cells.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1063/5.0076635
