Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83
Name
Rohrscheidt-Thymic CD4 T cell se.pdf
Size
2.19 MB
Format
Adobe PDF
Checksum (MD5)
f8bccb3a9f0b2d3b97435c6423129773
Author(s)
von Rohrscheidt, Julia
Petrozziello, Elisabetta
Nedjic, Jelena
Federle, Christine
Krzyzak, Lena
Ishido, Satoshi
Steinkasserer, Alexander
Klein, Ludger
Ploegh, Hidde
Date Issued
August 2016
Journal
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Citation
von Rohrscheidt, Julia et al. “Thymic CD4 T Cell Selection Requires Attenuation of March8-Mediated MHCII Turnover in Cortical Epithelial Cells through CD83.” The Journal of Experimental Medicine 213.9 (2016): 1685–1694.
Version
Final published version
Abstract
Deficiency of CD83 in thymic epithelial cells (TECs) dramatically impairs thymic CD4 T cell selection. CD83 can exert cell-intrinsic and –extrinsic functions through discrete protein domains, but it remains unclear how CD83’s capacity to operate through these alternative functional modules relates to its crucial role in TECs. In this study, using viral reconstitution of gene function in TECs, we found that CD83’s transmembrane domain is necessary and sufficient for thymic CD4 T cell selection. Moreover, a ubiquitination-resistant MHCII variant restored CD4 T cell selection in Cd83[superscript −/−] mice. Although during dendritic cell maturation CD83 is known to stabilize MHCII through opposing the ubiquitin ligase March1, regulation of March1 did not account for CD83’s TEC-intrinsic role. Instead, we provide evidence that MHCII in cortical TECs (cTECs) is targeted by March8, an E3 ligase of as yet unknown physiological substrate specificity. Ablating March8 in Cd83[superscript −/−] mice restored CD4 T cell development. Our results identify CD83-mediated MHCII stabilization through antagonism of March8 as a novel functional adaptation of cTECs for T cell selection. Furthermore, these findings suggest an intriguing division of labor between March1 and March8 in controlling inducible versus constitutive MHCII expression in hematopoietic antigen-presenting cells versus TECs.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1084/jem.20160316
