Autonomously designed free-form 2D DNA origami
Name
eaav0655.full.pdf
Size
2.35 MB
Format
Adobe PDF
Checksum (MD5)
572612f27bffd6d97861e1cdaace69cd
Author(s)
Zhang, Fei
Qi, Xiaodong
Yan, Hao
Jun, Hyungmin
Shepherd, Tyson R
Ratanalert, Sakul
Bathe, Mark
Date Issued
November 2018
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Jun, Hyungmin et al. “Autonomously Designed Free-Form 2D DNA Origami.” Science Advances 5, 1 (January 2019): eaav0655 © 2019 The Authors
Version
Final published version
Abstract
Scaffolded DNA origami offers the unique ability to organize molecules in nearly arbitrary spatial patterns at the nanometer scale, with wireframe designs further enabling complex 2D and 3D geometries with irregular boundaries and internal structures. The sequence design of the DNA staple strands needed to fold the long scaffold strand to the target geometry is typically performed manually, limiting the broad application of this materials design paradigm. Here, we present a fully autonomous procedure to design all DNA staple sequences needed to fold any free-form 2D scaffolded DNA origami wireframe object. Our algorithm uses wireframe edges consisting of two parallel DNA duplexes and enables the full autonomy of scaffold routing and staple sequence design with arbitrary network edge lengths and vertex angles. The application of our procedure to geometries with both regular and irregular external boundaries and variable internal structures demonstrates its broad utility for nanoscale materials science and nanotechnology.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/sciadv.aav0655
