mRNA stability in soma and neurites of cultured neuronal cells
Name
1003289754-MIT.pdf
Description
Full printable version
Size
3.26 MB
Format
Adobe PDF
Checksum (MD5)
fb1770ccdf61001630bb8ea249c3c40d
Author(s)
Srinath, Chetan
Advisor(s)
Christopher B. Burge.
Date Issued
2017
Publisher
Massachusetts Institute of Technology
Abstract
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To determine mRNA decay rates, we analyzed the subcellular transcriptomes of neural projections and soma of mouse neuronal cells following inhibition of transcription with actinomycin-D. Less stable transcripts were enriched for GU-rich elements in their 3' UTRs. Around 12% of alternative splicing isoform pairs differed in stability, and cassette alternative ("skipped") exons that negatively impact stability are enriched for A-rich sequences. Overall, decay rates were similar across soma and neurites. However, differences in stability between soma and neurites were observed for GC-rich alternative first exon isoforms, which were preferentially stabilized in neurites. Our results suggest that 5' UTRs may play a key role in regulating local mRNA stability in neurites.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2017.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 45-50).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
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