Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy
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Cjen-2010-Second-order susceptibility.pdf
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Author(s)
So, Peter T. C.
Chen, Wei-Liang
Li, Tsung-Hsian
Su, Ping-Jung
Chou, Chen-Kuan
Fwu, Peter Tramyeon
Dong, Chen-Yuan
Kim, Daekeun
Date Issued
February 2010
Journal
Proceedings of SPIE--the International Society for Optical Engineering; v. 7569
Publisher
SPIE
Citation
Wei-Liang Chen, Tsung-Hsian Li, Ping-Jung Su, Chen-Kuan Chou, Peter Tramyeon Fwu, Sung-Jan Lin, Daekeun Kim, Peter T. C. So, and Chen-Yuan Dong (2010). Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy. Proc. SPIE 7569, 75691P/1-7. ©2010 COPYRIGHT SPIE--The International Society for Optical Engineering
Version
Final published version
Abstract
Second harmonic generation (SHG) microscopy has become an important tool for minimally invasive biomedical imaging. However, differentiation of different second harmonic generating species is mainly provided by morphological features. Using excitation polarization-resolved SHG microscopy we determined the ratios of the second-order susceptibility tensor elements at single pixel resolution. Mapping the resultant ratios for each pixel onto an image provides additional contrast for the differentiation of different sources of SHG. We demonstrate this technique by imaging collagen-muscle junction of chicken wing.
Subjects
second harmonic generation
polarization
microscopy
collagen
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/12.843015
