An examination of peptide-tether binding to gold and glass surfaces
Name
558832029-MIT.pdf
Description
Full printable version
Size
1.97 MB
Format
Adobe PDF
Checksum (MD5)
17016b1798d4e0fdda606979a15d8903
Author(s)
Kunkel, Jacquelyn Anne
Advisor(s)
Matthew J. Lang.
Date Issued
2009
Publisher
Massachusetts Institute of Technology
Abstract
For many biological applications, it is beneficial to know that a peptide will bind to a surface. In this thesis, a tether, bead, peptide complex is constructed using the gold binding peptide (GBP) of sequence (CGGVSGSSPDS). Several assays and assay modifications are developed and tested to attempt to attach the GBP first to a gold slide and then to gold nanoparticles. Four peptides: FO₂, K1, K2, K3, known to bind to sapphire were attached to glass to see if it was possible to modify the sapphire assay to work with glass.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2009.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 14).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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