Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production
Name
sinskey3.pdf
Size
513.03 KB
Format
Adobe PDF
Checksum (MD5)
08f4d9495862f2f4f5be8677004807d5
Author(s)
Brigham, Christopher J.
Song, Yuyang
Matsumoto, Ken’ichiro
Yamada, Miwa
Gohda, Aoi
Taguchi, Seiichi
Sinskey, Anthony J
Date Issued
November 2011
Journal
Applied Microbiology and Biotechnology
Publisher
Springer-Verlag
Citation
Song, Yuyang et al. “Engineered Corynebacterium Glutamicum as an Endotoxin-free Platform Strain for Lactate-based Polyester Production.” Applied Microbiology and Biotechnology 93.5 (2011): 1917–1925.
Version
Author's final manuscript
Abstract
The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli (Taguchi et al. 2008). Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA), and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/s00253-011-3718-0
