CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
Author(s)
Chavez, Alejandro
Collins, James J.
Shapiro, Rebecca
Alternative Title
CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
Date Issued
March 2018
Journal
Nature Reviews Microbiology
Publisher
Nature Publishing Group
Citation
Shapiro, Rebecca S. et al. “CRISPR-Based Genomic Tools for the Manipulation of Genetically Intractable Microorganisms.” Nature Reviews Microbiology 16, 6 (March 2018): 333–339 © 2018 Macmillan Publishers Limited, part of Springer Nature
Version
Author's final manuscript
Abstract
Genetic manipulation of microorganisms has been crucial in understanding their biology, yet for many microbial species, robust tools for comprehensive genetic analysis were lacking until the advent of CRISPR–Cas-based gene editing techniques. In this Progress article, we discuss advances in CRISPR-based techniques for the genetic analysis of genetically intractable microorganisms, with an emphasis on mycobacteria, fungi and parasites. We discuss how CRISPR-based analyses in these organisms have enabled the discovery of novel gene functions, the investigation of genetic interaction networks and the identification of virulence factors.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Synthetic Biology Center
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41579-018-0002-7
