Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes
Name
1462-2920.15834.pdf
Description
Published version
Size
705.08 KB
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Unknown
Checksum (MD5)
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Author(s)
Biller, Steven J
Lundeen, Rachel A
Hmelo, Laura R
Becker, Kevin W
Arellano, Aldo A
Dooley, Keven
Heal, Katherine R
Carlson, Laura T
Van Mooy, Benjamin AS
Ingalls, Anitra E
Date Issued
November 2021
Journal
Environmental Microbiology
Publisher
Wiley
Citation
Biller, Steven J, Lundeen, Rachel A, Hmelo, Laura R, Becker, Kevin W, Arellano, Aldo A et al. 2022. "Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes." Environmental Microbiology, 24 (1).
Version
Final published version
Abstract
Extracellular vesicles are small (~50-200 nm diameter) membrane-bound structures released by cells from all domains of life. While vesicles are abundant in the oceans, their functions, both for cells themselves and the emergent ecosystem, remain a mystery. To better characterize these particles - a prerequisite for determining function - we analysed the lipid, protein, and metabolite content of vesicles produced by the marine cyanobacterium Prochlorococcus. We show that Prochlorococcus exports a diverse array of cellular compounds into the surrounding seawater enclosed within discrete vesicles. Vesicles produced by two different strains contain some materials in common, but also display numerous strain-specific differences, reflecting functional complexity within vesicle populations. The vesicles contain active enzymes, indicating that they can mediate extracellular biogeochemical reactions in the ocean. We further demonstrate that vesicles from Prochlorococcus and other bacteria associate with diverse microbes including the most abundant marine bacterium, Pelagibacter. Together, our data point toward hypotheses concerning the functional roles of vesicles in marine ecosystems including, but not limited to, possibly mediating energy and nutrient transfers, catalysing extracellular biochemical reactions, and mitigating toxicity of reactive oxygen species.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Woods Hole Oceanographic Institution
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1111/1462-2920.15834
