Reconstitution of Morphogen Signaling Gradients in Cultured Cells
Name
nihms-1693961.pdf
Description
Accepted version
Size
846.66 KB
Format
Unknown
Checksum (MD5)
aa3b753ed518c4134eb472b820e966e5
Author(s)
Kim, Julia S.
Pineda, Michael
Li, Pulin
Date Issued
December 2020
Publisher
Springer US
Citation
Kim, Julia S., Pineda, Michael and Li, Pulin. 2020. "Reconstitution of Morphogen Signaling Gradients in Cultured Cells." 2258.
Version
Final published version
Abstract
Development of multicellular organisms depends on the proper establishment of signaling information in space and time. Secreted molecules called morphogens form concentration gradients in space and provide positional information to differentiating cells within the organism. Although the key molecular components of morphogen pathways have been identified, how the architectures and key parameters of morphogen pathways control the properties of signaling gradients, such as their size, speed, and robustness to perturbations, remains challenging to study in developing embryos. Reconstituting morphogen gradients in cell culture provides an alternative approach to address this question. Here we describe the methodology for reconstituting Sonic Hedgehog (SHH) signaling gradients in mouse fibroblast cells. The protocol includes the design of morphogen sending and receiving cell lines, the setup of radial and linear gradients, the quantitative time-lapse imaging, and the data analysis. Similar approaches could potentially be applied to other cell–cell communication pathways.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/978-1-0716-1174-6_4
