The Human Kinetochore Ska1 Complex Facilitates Microtubule Depolymerization-Coupled Motility
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main article
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Author(s)
Welburn, Julie P. I.
Grishchuk, Ekaterina L.
Backer, Chelsea B.
Wilson-Kubalek, Elizabeth M.
Yates III, John R.
Cheeseman, Iain M
Date Issued
March 2009
Journal
Developmental Cell
Publisher
Cell Press
Citation
Welburn, Julie P.I. et al. “The Human Kinetochore Ska1 Complex Facilitates Microtubule Depolymerization-Coupled Motility.” Developmental Cell 16.3 (2009): 374-385.
Version
Author's final manuscript
Abstract
Mitotic chromosome segregation requires that kinetochores attach to microtubule polymers and harness microtubule dynamics to drive chromosome movement. In budding yeast, the Dam1 complex couples kinetochores with microtubule depolymerization. However, a metazoan homolog of the Dam1 complex has not been identified. To identify proteins that play a corresponding role at the vertebrate kinetochore-microtubule interface, we isolated a three subunit human Ska1 complex, including the previously uncharacterized protein Rama1 that localizes to the outer kinetochore and spindle microtubules. Depletion of Ska1 complex subunits severely compromises proper chromosome segregation. Reconstituted Ska1 complex possesses two separable biochemical activities: direct microtubule binding through the Ska1 subunit, and microtubule-stimulated oligomerization imparted by the Rama1 subunit. The full Ska1 complex forms assemblies on microtubules that can facilitate the processive movement of microspheres along depolymerizing microtubules. In total, these results demonstrate a critical role for the Ska1 complex in interacting with dynamic microtubules at the outer kinetochore.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.devcel.2009.01.011
