Femtomolar detection of SARS-CoV-2 via peptide beacons integrated on a miniaturized TIRF microscope
Name
sciadv.abn2378.pdf
Description
Published version
Size
1.97 MB
Format
Adobe PDF
Checksum (MD5)
54506503de5de5ef23999344a07b6567
Author(s)
Tripathy, Soumya P
Ponnapati, Manvitha
Bhat, Suhaas
Jacobson, Joseph
Chatterjee, Pranam
Date Issued
August 26, 2022
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Tripathy, Soumya P, Ponnapati, Manvitha, Bhat, Suhaas, Jacobson, Joseph and Chatterjee, Pranam. 2022. "Femtomolar detection of SARS-CoV-2 via peptide beacons integrated on a miniaturized TIRF microscope." Science Advances, 8 (34).
Version
Final published version
Abstract
The novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) continues to pose a substantial global health threat. Along with vaccines and targeted therapeutics, there is a critical need for rapid diagnostic solutions. In this work, we use computational protein modeling tools to suggest molecular beacon architectures that function as conformational switches for high-sensitivity detection of the SARS-CoV-2 spike protein receptor binding domain (S-RBD). Integrating these beacons on a miniaturized total internal reflection fluorescence (mini-TIRF) microscope, we detect the S-RBD and pseudotyped SARS-CoV-2 with limits of detection in the femtomolar range. We envision that our designed mini-TIRF platform will serve as a robust platform for point-of-care diagnostics for SARS-CoV-2 and future emergent viral threats.
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Massachusetts Institute of Technology. Center for Bits and Atoms
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1126/sciadv.abn2378
