Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement
Name
Murrell-2011-Substrate Viscosity.pdf
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1.65 MB
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Author(s)
Murrell, Michael
Kamm, Roger Dale
Matsudaira, Paul T.
Date Issued
July 2011
Journal
Biophysical Journal
Publisher
Elsevier
Citation
Murrell, Michael, Roger Kamm, and Paul Matsudaira. “Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement.” Biophysical Journal 101, no. 2 (July 2011): 297–306. © 2011 Biophysical Society
Version
Final published version
Abstract
The movement of the epithelium plays vital roles in the development and renewal of complex tissues, from the separation of tissues in the early embryo, to turnover in the homeostasis of the gastrointestinal mucosa. Yet, despite its importance, a clear interpretation of the mechanism for collective motion in epithelial sheets remains elusive. This interpretation is prohibited by the lack of understanding of the relationship between motion and cell-cell contact, and their mediation by the mechanical properties of the underlying substrate. To better mimic physiological substrates that have inherent viscosity, we probe this relationship using polydimethylsiloxane, a substrate whose mechanical properties can be tuned from predominantly elastic to viscous by altering its cross-linking content. We therefore characterize the comparative spatiotemporal correlations in cell velocity during the movement of an epithelial monolayer as a function of the viscoelasticity of the substrate. Our results show that high correlation in cell velocity is achieved when the substrate G″(ω) is ∼0.4 × G′(ω). This correlation is driven by a balance between cell-cell contact and the adhesion and contraction of the extracellular matrix. For G″(ω) > G′(ω), this balance shifts, and contraction of the tissue drives the substrate to flow, further elevating the correlation in movement.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Mechanical Engineering
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2011.05.048
