DNA-loop-extruding SMC complexes can traverse one another in vivo
Name
DNA-loop_extruding_SMC_complexes_can_traverse_one_.pdf
Description
Accepted version
Size
3.74 MB
Format
Adobe PDF
Checksum (MD5)
70984b93752c9ea6abf3ec145407a774
Author(s)
Brandão, Hugo B
Ren, Zhongqing
Karaboja, Xheni
Mirny, Leonid A
Wang, Xindan
Date Issued
August 2021
Journal
Nature Structural & Molecular Biology
Publisher
Springer Science and Business Media LLC
Citation
Brandão, Hugo B, Ren, Zhongqing, Karaboja, Xheni, Mirny, Leonid A and Wang, Xindan. 2021. "DNA-loop-extruding SMC complexes can traverse one another in vivo." Nature Structural & Molecular Biology, 28 (8).
Version
Original manuscript
Abstract
Chromosome organization mediated by structural maintenance of chromosomes (SMC) complexes is vital in many organisms. SMC complexes act as motors that extrude DNA loops, but it remains unclear what happens when multiple complexes encounter one another on the same DNA in living cells and how these interactions may help to organize an active genome. We therefore created a crash-course track system to study SMC complex encounters in vivo by engineering defined SMC loading sites in the Bacillus subtilis chromosome. Chromosome conformation capture (Hi-C) analyses of over 20 engineered strains show an amazing variety of chromosome folding patterns. Through three-dimensional polymer simulations and theory, we determine that these patterns require SMC complexes to bypass each other in vivo, as recently seen in an in vitro study. We posit that the bypassing activity enables SMC complexes to avoid traffic jams while spatially organizing the genome.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41594-021-00626-1
