Fungal recognition is mediated by the association of dectin-1 and galectin-3 in macrophages
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Esteban-2011-Aug-Fungal recognition i.pdf
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Author(s)
Esteban, Alexandre
Vyas, Valmik K.
Strijbis, Karin
Ploegh, Hidde
Popp, Maximilian W.L.
Fink, Gerald R
Date Issued
August 2011
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Esteban, A. et al. “Fungal Recognition Is Mediated by the Association of Dectin-1 and Galectin-3 in Macrophages.” Proceedings of the National Academy of Sciences 108.34 (2011): 14270–14275.
Version
Final published version
Abstract
Dectin-1, the major β-glucan receptor in leukocytes, triggers an effective immune response upon fungal recognition. Here we use sortase-mediated transpeptidation, a technique that allows placement of a variety of probes on a polypeptide backbone, to monitor the behavior of labeled functional dectin-1 in live cells with and without fungal challenge. Installation of probes on dectin-1 by sortagging permitted highly specific visualization of functional protein on the cell surface and its subsequent internalization upon ligand presentation. Retrieval of sortagged dectin-1 expressed in macrophages uncovered a unique interaction between dectin-1 and galectin-3 that functions in the proinflammatory response of macrophages to pathogenic fungi. When macrophages expressing dectin-1 are exposed to Candida albicans mutants with increased exposure of β-glucan, the loss of galectin-3 dramatically accentuates the failure to trigger an appropriate TNF-α response.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1111415108
